Modeling electroporation in a single cell.
نویسندگان
چکیده
Electroporation uses electric pulses to promote delivery of DNA and drugs into cells. This study presents a model of electroporation in a spherical cell exposed to an electric field. The model determines transmembrane potential, number of pores, and distribution of pore radii as functions of time and position on the cell surface. For a 1-ms, 40 kV/m pulse, electroporation consists of three stages: charging of the cell membrane (0-0.51 micros), creation of pores (0.51-1.43 micros), and evolution of pore radii (1.43 micros to 1 ms). This pulse creates approximately 341,000 pores, of which 97.8% are small ( approximately 1 nm radius) and 2.2% are large. The average radius of large pores is 22.8 +/- 18.7 nm, although some pores grow to 419 nm. The highest pore density occurs on the depolarized and hyperpolarized poles but the largest pores are on the border of the electroporated regions of the cell. Despite their much smaller number, large pores comprise 95.3% of the total pore area and contribute 66% to the increased cell conductance. For stronger pulses, pore area and cell conductance increase, but these increases are due to the creation of small pores; the number and size of large pores do not increase.
منابع مشابه
Optimal Electroporation Condition for Small Interfering RNA Transfection into MDA-MB-468 Cell Line
Background: Electroporation is a valuable tool for small interfering RNA (siRNA) delivery into cells because it efficiently transforms a wide variety of cell types. Since electroporation condition for each cell type must be determined experimentally, this study presents an optimal electroporation strategy to reproducibly and efficiently transfect MDA-MB 468 human breast cancer cell with siRNA. ...
متن کاملEffect of Cell Size and Shape on Electric Field Threshold and Critical Transmembrane Voltage for Electroporation
Introduction: Electroporation is a technique for increasing the permeability of the cell membrane to otherwise non-permeate molecules due to an external electric field. This permeability enhancement is detectable if the induced transmembrane voltage becomes greater than a critical value which depends on the pulse strength threshold. In this study, the variabil...
متن کاملThe Endothelial Permeability Increased by Low Voltage and High Frequency Electroporation
Propose: Standard electroporation and electrochemotherapy caused the endothe- lial cell permeability and reduction in tumor blood flow. The effects of low voltage and high frequency electroporation on the endothelial cells permeability and viability were expected. Therefore, the propose of present study was to evaluate the effect of electroporation with bleomycin or alone on viability and perme...
متن کاملRapid Delivery of Gold Nanoparticles into Colon Cancer HT-29 Cells by Electroporation: In-vitro Study
Background: Electroporation has become a routine technique for rapid drug delivery for the treatment of cancer. Because of its simplicity and wide range of application, it has been applied for the transfer of gold-nanoparticles and can facilitate entry into target cancer cells. Objective: The aim of this study is finding optimal conditions in order to obtain high GNPs- uptake and cell via...
متن کاملA Quantitative Study of the Effect of Electroporation on the Electropermeability and Cell Survival
Introduction: Electroporation is a practical technique used to transport the molecules across the cell membrane. The utilization of fluorescent molecules is the method widely used to evaluate the electropermeability of cell membrane as a result of pulse application. It is also possible to use mathematical methods to predict the changes caused in cell electropermeability as a result of the chang...
متن کاملA Multi Objective Genetic Algorithm (MOGA) for Optimizing Thermal and Electrical Distribution in Tumor Ablation by Irreversible Electroporation
Background: Irreversible electroporation (IRE) is a novel tumor ablation technique. IRE is associated with high electrical fields and is often reported in conjunction with thermal damage caused by Joule heating. For good response to surgery it is crucial to produce minimum thermal damage in both tumoral and healthy tissues named Non-Thermal Irreversible Electroporation(NTIRE). Non-thermal irrev...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Biophysical journal
دوره 92 2 شماره
صفحات -
تاریخ انتشار 2007